Analyzer for biotechnology, PPSQ-51A, Shimadzu

Protein sequencer for laboratories

Devices of the PPSQ series, using automatic Edman degradation of proteins and peptides in combination with HPLC separation and highly sensitive detection of reaction products, as well as powerful software for analyzing the results, provide fast and reliable determination of amino acid sequences. The models differ in the number of reactors – 1 reactor (PPSQ-51A) or 3 reactors (PPSQ-53A).

Principles of work

Protein sequencer – equipment for determining the sequence of amino acids from the N-end to 50-60 amino acid residues. The basis of determining the amino acid sequence is Edman’s method – one of the early methods of determining the primary sequence (sequencing) of peptides. The essence of the method is to treat the investigated peptide with a certain set of reagents, which leads to the cleavage of one amino acid from the N-end of the sequence in the form of its phenylthiohydantoin derivative (Edman reaction). Identification of phenylthiohydantoin derivatives is performed using liquid chromatography under pressure.

Despite the disadvantages of this method (high labor and time costs) and the development of mass spectrometry, it is still used due to the stability and unequivocalness of the results obtained.